Original Article
Sarcoplasmic/Endoplasmic Reticulum Ca²⁺ ATPase-1 (SERCA-1) is a P-type ATPase responsible for maintaining intracellular calcium homeostasis by transporting Ca²⁺ ions from the cytoplasm into the sarcoplasmic reticulum. Ca²⁺ regulation plays a vital role in excitation–contraction coupling, muscle relaxation, cellular metabolism, and thermogenesis. Different expression of SERCA isoforms has been reported in muscle tissues. But comparative expression profiling of SERCA-1 across diverse rabbit muscle tissues remains insufficient. The present study aims to comparatively assess the gene expression profile of SERCA-1 in different rabbit muscle tissues to understand tissue-specific variation associated with functional and metabolic requirements.
Total RNA was isolated from six rabbit muscle tissues which included tibialis anterior (TA), extensor digitorum longus (EDL), soleus, trapezius, gastrocnemius (GCN), and ventricle using TRIzol reagent. RNA integrity was confirmed by agarose gel electrophoresis and quantified using spectrophotometry. Complementary DNA (cDNA) was synthesized using reverse transcription, followed by amplification through real-time quantitative PCR (RT-qPCR) using gene-specific primers for SERCA-1 with 18S rRNA as control. Relative expression levels were analyzed based on amplification and melt curve profiles.
SERCA-1 expression was identified in all six muscle tissues, showing differential transcriptional regulation. The melt curve analysis confirmed primer specificity, indicating amplification of target gene sequences. The study provides insight into Ca²⁺ regulatory mechanisms in skeletal muscle physiology and provides details for further investigation into muscle metabolism and pathophysiological conditions.
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