Actinomycetes synthesized silver nanoparticles: characterization and its theraputic uses

Nanoparticles define a major role in plant disease management and therapeutic applications due to their physical attributes. Because of their high surface to bulk silver atom ratio, silver is primarily made up of silver oxide. Strong antibacterial, antifungal, antiviral, and anti-inflammatory properties have been demonstrated by silver nanoparticles. Silver nanoparticles were created using a straightforward, affordable, and environmentally friendly biosynthetic process. The goal of this study was to compare silver nanoparticles made by chemical and microbiological processes and their therapeutic applications by using a pathogenic microorganism. The Actinomycetes sp. was used to create silver nanoparticles by a microbiological process and chemical synthesis by chemical reduction of silver ions (Ag+) with the help of sodium borohydride or sodium citrate. The silver nanoparticles that were produced using Actinomycetes and the obtained silver nanoparticles from chemical synthesis were used for characterization studied using UV-vis spectroscopy, FTIR and SEM. SEM images of the silver nanoparticles had sizes of 70nm -110 nm. In FTIR Analysis all the nanoparticles showed the same functional group of (-OH) ranges between 3000 to 3500 Wavenumber per cm, the Amine(-NH2) functional group attached between the 2000 to 2500 Wavenumber per cm and the carboxyl(-COOH) functional group attached between 1000 to 2000 Wavenumber per cm. In the UV-vis spectroscopy the presence of silver metal was confirmed the range between 200nm to 250nm. The synthesised silver nanoparticles showed the resistance against the pathogens like E. coli, Klebsiella sp., Candida sp., Pseudomonas sp. and Staphylococcus aureus. This antibacterial activity was conducted using Muller Hinton agar media by well diffusion method.